Investigating the Role of Latrophilin Adhesion G-protein Coupled Receptors in Neural Circuit Patterning
Development of genetic mouse models to study Latrophilin gene function - Visualizing Latrophilin protein via incorporation of mVenus fluorescent protein
Using patch-clamp electrophysiology methods, we are able to isolate individual neurons in the brain and study their fundamental properties: action potentials, synaptic transmission, morphological architecture.
Single Neuron Analysis
Visualizing Synaptic Contacts
Biocytin loaded neuronal dendrite (red)
with Latrophilin marked synapses (green)
Latrophilin Synaptic Contacts
3D Neuron Reconstruction & Analysis
Biocytin loaded neuronal dendrite (red) with spines (blue) digitally reconstructed in 3-dimensions. Latrophilin marked synapses (green) in contact with neuron of interest.